The Application and Development of SILAC Technology in Proteomics

The Application and Development of SILAC Technology in Proteomics

Stable isotope labeling with amino acids is the new technology that is used for halo analysis of protein expression by taking advantage of stable isotope labeling amino acid binding mass spectrometry in the cell culture process. It can provide support for qualitative analysis of the protein, also offer accurate quantitative analysis with fewer sample requirements, but the simple and efficient process.

SHIRLEY – Sep 30th, 2017 – Creative Proteomics, which specializes in a full range of services to support various proteome-related researches from identification of single proteins to large-scale proteomic studies. Besides, we also offer professional products to support further researches and studies. Such as the Stable Isotope Standard, that will be helpful for the proteomics research use.

Stable isotope labeling with amino acids is the new technology that is used for halo analysis of protein expression by taking advantage of stable isotope labeling amino acid binding mass spectrometry in the cell culture process. It can provide support for qualitative analysis of the protein, also offer accurate quantitative analysis with fewer sample requirements, but the simple and efficient process.

The applications and specific strategies of SILAC technology are in the continuous development of updates. There are many applications and improvements, such as the differential expression of proteins, especially the identification of tumor molecular markers, protein post-translational modification, drug proteome, protein interaction and signal path, along with other directions.

At present, SILAC technology is mainly used as one of the main strategies to find biomarkers of disease/tumor by studying the differential proteins between the different state of cells and tissues, along with different state of tissues. SILAC, combining with MS technology can identify more low abundance protein marker molecules and target proteins, which have great potential for tumor marker screening.

SILAC technique facilitates the identification of post-translational proteins and the determination of modification sites. Quantitative proteomics methods for stable isotope labeling Identify the occurrence and site of phosphorylation / dephosphorylation by differences in the abundance of characteristic isotope peptides, which have a significant advantage in protein phosphorylation analysis. SILAC combined with immunoprecipitation and other biochemical means can not only identify the signal pathway in the bovine phosphorylated protein components but reveal the new signal regulatory molecules. In addition to its application in the phosphorylation site identification and signaling pathways, SILAC and affinity chromatography enrichment techniques have also been successfully used for the analysis of glycosylation and methylation of proteins.

The interaction of protein-protein with SILAC combined with MS technique can overcome the limitation of yeast two-hybrid method, it can effectively distinguish the background (false positive) from the real interaction protein, detect weak or unstable protein interaction. Meanwhile, this technology can support real-time analysis of the dynamic changes in protein interaction.

Currently, SILAC-based quantitative proteomics techniques have been used in drug resistance mechanisms and drug therapy target molecules and other related research. Besides, SILAC technology being successfully applied in subcellular organelle proteomics, plant proteomics, and other fields. Researchers used SILAC to study the dynamic changes of nucleolus proteins in cells and demonstrated the feasibility of SILAC technology in nuclear proteomics research with fluorescence microscopy.

As an important method of sequencing proteomics, SILAC is still in constant development and innovation. However, it has developed from the initial limited to the analysis of cultured cell protein expression, extended to the cells, the group longitudinal, tissue protein expression and protein interaction between the multi-field applications. At the same time, it provides a platform for researchers to study the state of life, especially the pathological state. In particular, researchers have found a number of candidate tumor markers by SILAC which also offer the scientific basis for clinical treatment of the tumor. Although SILAC technology still has some limitations, it is impossible to solve all the problems in the study, we can expect the further application in more fields with continuous development.

About Stable Isotope labeling:

Creative Proteomics offers Stable Isotope Labeled Analytical Standard for proteomics research use:

Analytical Standard
Biological Standards
Drug analytical Standards
SILAC Kits and Reagents

Media Contact
Company Name: Creative Proteomics
Contact Person: Mandy Scott
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Website: http://www.creative-proteomics.com

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